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Jackson Laboratory ift88 floxed allele
Ift88 Floxed Allele, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ift88+floxed+allele/pm41951597-176-10-13?v=Jackson+Laboratory
Average 86 stars, based on 1 article reviews

ift88 floxed allele - by Bioz Stars, 2026-07

86/100 stars

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(A) Primary cilia were visualized by ACIII (green) in the VMH of WT and IFT88-KOSF-1 mice. Scale bar: 10 μm. (B) Length and percentage of the VMH cilia shown in A. **P < 0.01; ***P < 0.001, Student’s t test. (C) Left: weekly body weight of WT and IFT88-KOSF-1 mice fed on NC. Right: representative photographs of 18-week-old male mice on NC diet. *P < 0.05, 2-way ANOVA. (D) Left: fat and lean mass were measured by NMR. Right: representative DEXA image. **P < 0.01, Student’s t test. (E) Representative s.c.WAT and gWAT from WT and IFT88-KOSF-1 mice. Scale bars: 0.5 mm. (F and G) H&E staining (F) and adipocyte cell surface (G) of gWAT. Adipocyte cell surface measured by ImageJ. Scale bar: 50 μm. ***P < 0.001, 2-way ANOVA. (H) Serum leptin levels in WT and IFT88-KOSF-1 mice at 14 week of age. **P < 0.01, Student’s t test. (I) Left: schematic showing bilateral viral injection into VMH. Right: schematic for AAV2-GFP and AAV2-Cre-GFP constructs used for viral injection (upper) and validation images of primary cilia KD in VMH (lower). Scale bars: 0.1 mm (yellow); 10μm (white). (J) Weekly body weight after injection with AAV2-GFP or AAV2-Cre-GFP into the VMH. ***P < 0.001, 2-way ANOVA. (K) Left: body compositions 10 weeks after injection of AAV2-GFP or AAV2-Cre-GFP. Right: representative DEXA images. ***P < 0.001, Student’s t test. Number of animals examined is expressed in parentheses in each graph. Results are expressed as mean ± SD.

Journal: The Journal of Clinical Investigation

Article Title: Ventromedial hypothalamic primary cilia control energy and skeletal homeostasis

doi: 10.1172/JCI138107

Figure Lengend Snippet: (A) Primary cilia were visualized by ACIII (green) in the VMH of WT and IFT88-KOSF-1 mice. Scale bar: 10 μm. (B) Length and percentage of the VMH cilia shown in A. **P < 0.01; ***P < 0.001, Student’s t test. (C) Left: weekly body weight of WT and IFT88-KOSF-1 mice fed on NC. Right: representative photographs of 18-week-old male mice on NC diet. *P < 0.05, 2-way ANOVA. (D) Left: fat and lean mass were measured by NMR. Right: representative DEXA image. **P < 0.01, Student’s t test. (E) Representative s.c.WAT and gWAT from WT and IFT88-KOSF-1 mice. Scale bars: 0.5 mm. (F and G) H&E staining (F) and adipocyte cell surface (G) of gWAT. Adipocyte cell surface measured by ImageJ. Scale bar: 50 μm. ***P < 0.001, 2-way ANOVA. (H) Serum leptin levels in WT and IFT88-KOSF-1 mice at 14 week of age. **P < 0.01, Student’s t test. (I) Left: schematic showing bilateral viral injection into VMH. Right: schematic for AAV2-GFP and AAV2-Cre-GFP constructs used for viral injection (upper) and validation images of primary cilia KD in VMH (lower). Scale bars: 0.1 mm (yellow); 10μm (white). (J) Weekly body weight after injection with AAV2-GFP or AAV2-Cre-GFP into the VMH. ***P < 0.001, 2-way ANOVA. (K) Left: body compositions 10 weeks after injection of AAV2-GFP or AAV2-Cre-GFP. Right: representative DEXA images. ***P < 0.001, Student’s t test. Number of animals examined is expressed in parentheses in each graph. Results are expressed as mean ± SD.

Article Snippet: To generate experimental mice, IFT88-KO SF-1 male mice heterozygous for the SF-1–Cre transgene and homozygous for the floxed Ift88 allele (Ift88 fl/fl ) were crossed with female mice (Jackson Laboratory stock no. 022409) homozygous for the floxed Ift88 allele (Ift88 fl/fl ).

Techniques: Staining, Injection, Construct, Biomarker Discovery

(A) Comparable body weights of WT and IFT88-KOSF-1 littermates at 12 week of age. (B) Cumulative daily food intake on NC diet. (C–E) Temporal changes of VO2 (C), VCO2 (D), and EE (E) in WT and IFT88-KOSF-1 littermates. *P < 0.05 (red asterisks), 2-way ANOVA; **P < 0.01, Student’s t test. (F) Hypothalamic Pomc, Agrp, and Npy expression. *P < 0.05, Student’s t test. (G) Temporal changes of movement in WT and IFT88-KOSF-1 littermates. (H) Morphology and H&E staining of the iBAT from WT and IFT88-KOSF-1 littermates. Scale bar, 0.5mm for yellow and 50μm for black. (I) Mitochondria DNA contents in iBAT. **P < 0.01, Student’s t test. (J) Immunohistochemistry for UCP1 in iBAT of WT and IFT88-KOSF-1 mice. Scale bar: 100μm. (K and L) Western blot (K) and relative fold changes of protein levels (L) for UCP1. ***P < 0.001, Student’s t test. (M) Relative gene expression levels in iBAT. *P < 0.05; **P < 0.01; ***P < 0.001, Student’s t test. (N) Age-dependent serum NE levels in WT and IFT88-KOSF-1 mice. *P < 0.05; **P < 0.01, 2-way ANOVA. (O) Correlation analysis between serum NE and body weight of WT and IFT88-KOSF-1 mice. Number of animals examined is expressed parentheses in each graph. Results are expressed as mean ± SD.

Journal: The Journal of Clinical Investigation

Article Title: Ventromedial hypothalamic primary cilia control energy and skeletal homeostasis

doi: 10.1172/JCI138107

Figure Lengend Snippet: (A) Comparable body weights of WT and IFT88-KOSF-1 littermates at 12 week of age. (B) Cumulative daily food intake on NC diet. (C–E) Temporal changes of VO2 (C), VCO2 (D), and EE (E) in WT and IFT88-KOSF-1 littermates. *P < 0.05 (red asterisks), 2-way ANOVA; **P < 0.01, Student’s t test. (F) Hypothalamic Pomc, Agrp, and Npy expression. *P < 0.05, Student’s t test. (G) Temporal changes of movement in WT and IFT88-KOSF-1 littermates. (H) Morphology and H&E staining of the iBAT from WT and IFT88-KOSF-1 littermates. Scale bar, 0.5mm for yellow and 50μm for black. (I) Mitochondria DNA contents in iBAT. **P < 0.01, Student’s t test. (J) Immunohistochemistry for UCP1 in iBAT of WT and IFT88-KOSF-1 mice. Scale bar: 100μm. (K and L) Western blot (K) and relative fold changes of protein levels (L) for UCP1. ***P < 0.001, Student’s t test. (M) Relative gene expression levels in iBAT. *P < 0.05; **P < 0.01; ***P < 0.001, Student’s t test. (N) Age-dependent serum NE levels in WT and IFT88-KOSF-1 mice. *P < 0.05; **P < 0.01, 2-way ANOVA. (O) Correlation analysis between serum NE and body weight of WT and IFT88-KOSF-1 mice. Number of animals examined is expressed parentheses in each graph. Results are expressed as mean ± SD.

Techniques: Expressing, Staining, Immunohistochemistry, Western Blot, Gene Expression

(A) Cumulative rebound food intake of 10-week-old littermates after overnight fasting for 18 hours. *P < 0.05, 2-way ANOVA. (B) Rebound food intake of 12-week-old littermates after leptin administration. **P < 0.01, 2-way ANOVA. (C and D) Western blot (C) and relative fold changes of protein levels (D) for hypothalamic STAT3 in WT and IFT88-KOSF-1 mice. (E) Immunohistochemical analysis of p-STAT3 activation after leptin injection. Ten-week-old WT and IFT88-KOSF-1 mice were used for analysis. Note that p-STAT3–positive cells are specifically decreased in VMH. Scale bar: 100 μm. (F) Relative p-STAT3 expression in VMH or ARC. ***P < 0.001, Student’s t test. (G) Body weight for 12-week-old WT and IFT88-KOSF-1 littermates used for metabolic cage study shown in (H–K). (H) Cumulative food intake after leptin administration measured in metabolic chamber. **P < 0.01, 2-way ANOVA. (I–K) Temporal change of VO2 and its average (I), VCO2 and its average (J), and EE and its average (K). *P < 0.05, Student’s t test. Number of animals examined is expressed in parentheses in each graph. Data are expressed as mean ± SD.

Journal: The Journal of Clinical Investigation

Article Title: Ventromedial hypothalamic primary cilia control energy and skeletal homeostasis

doi: 10.1172/JCI138107

Figure Lengend Snippet: (A) Cumulative rebound food intake of 10-week-old littermates after overnight fasting for 18 hours. *P < 0.05, 2-way ANOVA. (B) Rebound food intake of 12-week-old littermates after leptin administration. **P < 0.01, 2-way ANOVA. (C and D) Western blot (C) and relative fold changes of protein levels (D) for hypothalamic STAT3 in WT and IFT88-KOSF-1 mice. (E) Immunohistochemical analysis of p-STAT3 activation after leptin injection. Ten-week-old WT and IFT88-KOSF-1 mice were used for analysis. Note that p-STAT3–positive cells are specifically decreased in VMH. Scale bar: 100 μm. (F) Relative p-STAT3 expression in VMH or ARC. ***P < 0.001, Student’s t test. (G) Body weight for 12-week-old WT and IFT88-KOSF-1 littermates used for metabolic cage study shown in (H–K). (H) Cumulative food intake after leptin administration measured in metabolic chamber. **P < 0.01, 2-way ANOVA. (I–K) Temporal change of VO2 and its average (I), VCO2 and its average (J), and EE and its average (K). *P < 0.05, Student’s t test. Number of animals examined is expressed in parentheses in each graph. Data are expressed as mean ± SD.

Techniques: Western Blot, Immunohistochemical staining, Activation Assay, Injection, Expressing

(A and B) Blood glucose (A) and insulin (B) levels in WT and IFT88-KOSF-1 littermates at 12 week of age. *P < 0.05, Student’s t test. (C) Left: GTT between WT and IFT88-KOSF-1. Right: AUC from GTT. *P < 0.05; **P < 0.01, 2-way ANOVA. (D) Left: ITT in indicated genotypes. Right: AUC for the ITT. *P < 0.05; **P < 0.01; ***P < 0.001, 2-way ANOVA. (E) Glucose-stimulated insulin secretion (GSIS) during GTT experiments. (F) Relative expression of gluconeogenic genes in the liver. **P < 0.01; ***P < 0.001, Student’s t test. (G) H&E staining of liver from WT and IFT88-KOSF-1 mice. Scale bar: 50μm. (H) Relative expression of lipogenesis-related genes in the liver. *P < 0.05; **P < 0.01; ***P < 0.001, Student’s t test. (I) GTT (left) and its AUC (right) in viral-mediated VMH-specific IFT88 KD models. *P < 0.05, 2-way ANOVA. (J) ITT (left) and its AUC (right) in viral-mediated VMH-specific IFT88 KD models. *P < 0.05; **P < 0.01, 2-way ANOVA. Number of animals examined is expressed parentheses in each graph. Data are expressed as mean ± SD.

Journal: The Journal of Clinical Investigation

Article Title: Ventromedial hypothalamic primary cilia control energy and skeletal homeostasis

doi: 10.1172/JCI138107

Figure Lengend Snippet: (A and B) Blood glucose (A) and insulin (B) levels in WT and IFT88-KOSF-1 littermates at 12 week of age. *P < 0.05, Student’s t test. (C) Left: GTT between WT and IFT88-KOSF-1. Right: AUC from GTT. *P < 0.05; **P < 0.01, 2-way ANOVA. (D) Left: ITT in indicated genotypes. Right: AUC for the ITT. *P < 0.05; **P < 0.01; ***P < 0.001, 2-way ANOVA. (E) Glucose-stimulated insulin secretion (GSIS) during GTT experiments. (F) Relative expression of gluconeogenic genes in the liver. **P < 0.01; ***P < 0.001, Student’s t test. (G) H&E staining of liver from WT and IFT88-KOSF-1 mice. Scale bar: 50μm. (H) Relative expression of lipogenesis-related genes in the liver. *P < 0.05; **P < 0.01; ***P < 0.001, Student’s t test. (I) GTT (left) and its AUC (right) in viral-mediated VMH-specific IFT88 KD models. *P < 0.05, 2-way ANOVA. (J) ITT (left) and its AUC (right) in viral-mediated VMH-specific IFT88 KD models. *P < 0.05; **P < 0.01, 2-way ANOVA. Number of animals examined is expressed parentheses in each graph. Data are expressed as mean ± SD.

Techniques: Expressing, Staining

(A–D) DEXA analyses were performed at indicated ages and measured bone parameters. BMD (A), BMC (B), BV (C), and BA (D) levels of WT and IFT88-KOSF-1 mice. *P < 0.05; **P < 0.01, 2-way ANOVA. (E–H) Correlation between serum NE and BMD (E), BMC (F), BV (G), and BA (H) respectively. For correlation testing, linear regression models in R were utilized and R2 values were reported. (I) Femur NE level of WT and IFT88-KOSF-1 littermates at 16 weeks of age. ***P < 0.001, Student’s t test. (J) Representative μCT images of the femurs. The 12-week old WT and IFT88-KOSF-1 littermates were used for analyses. (K) BMD, BV/TV, BS, Tb.Th., Tb.N., and Tb.Sp. were analyzed using μCT from J. *P < 0.05; **P < 0.01, Student’s t test. (L) Representative μCT images of femurs in control (AAV2-GFP) and viral-mediated VMH-specific primary cilia KD (AAV2-Cre-GFP) mice. (M) BMD, BV/TV, BS, Tb.Th., Tb.N., and Tb.Sp. were analyzed using μCT from L. *P < 0.05, Student’s t test. Number of animals examined is expressed parentheses in each graph. Data are expressed as mean ± SD.

Journal: The Journal of Clinical Investigation

Article Title: Ventromedial hypothalamic primary cilia control energy and skeletal homeostasis

doi: 10.1172/JCI138107

Figure Lengend Snippet: (A–D) DEXA analyses were performed at indicated ages and measured bone parameters. BMD (A), BMC (B), BV (C), and BA (D) levels of WT and IFT88-KOSF-1 mice. *P < 0.05; **P < 0.01, 2-way ANOVA. (E–H) Correlation between serum NE and BMD (E), BMC (F), BV (G), and BA (H) respectively. For correlation testing, linear regression models in R were utilized and R2 values were reported. (I) Femur NE level of WT and IFT88-KOSF-1 littermates at 16 weeks of age. ***P < 0.001, Student’s t test. (J) Representative μCT images of the femurs. The 12-week old WT and IFT88-KOSF-1 littermates were used for analyses. (K) BMD, BV/TV, BS, Tb.Th., Tb.N., and Tb.Sp. were analyzed using μCT from J. *P < 0.05; **P < 0.01, Student’s t test. (L) Representative μCT images of femurs in control (AAV2-GFP) and viral-mediated VMH-specific primary cilia KD (AAV2-Cre-GFP) mice. (M) BMD, BV/TV, BS, Tb.Th., Tb.N., and Tb.Sp. were analyzed using μCT from L. *P < 0.05, Student’s t test. Number of animals examined is expressed parentheses in each graph. Data are expressed as mean ± SD.

Techniques: Control

(A–D) Changes of bone parameters BMD (A), BMC (B), BV (C), and BA (D) in both WT and IFT88-KOSF-1 littermates before and after i.p. injection of ISO (15 mg/kg) for 2 weeks. Bone parameters measured using DEXA analysis. *P < 0.05; **P < 0.01, 2-way ANOVA. (E) Representative femur images of WT and IFT88-KOSF-1 littermates after ISO administration. (F) The BMD, BV/TV, BS, Tb.Th., Tb.N., and Tb.Sp. were analyzed using μCT in WT and IFT88-KOSF-1 mice given ISO. Number of animals examined is expressed in parentheses in each graph. Results are expressed as mean ± SD.

Journal: The Journal of Clinical Investigation

Article Title: Ventromedial hypothalamic primary cilia control energy and skeletal homeostasis

doi: 10.1172/JCI138107

Figure Lengend Snippet: (A–D) Changes of bone parameters BMD (A), BMC (B), BV (C), and BA (D) in both WT and IFT88-KOSF-1 littermates before and after i.p. injection of ISO (15 mg/kg) for 2 weeks. Bone parameters measured using DEXA analysis. *P < 0.05; **P < 0.01, 2-way ANOVA. (E) Representative femur images of WT and IFT88-KOSF-1 littermates after ISO administration. (F) The BMD, BV/TV, BS, Tb.Th., Tb.N., and Tb.Sp. were analyzed using μCT in WT and IFT88-KOSF-1 mice given ISO. Number of animals examined is expressed in parentheses in each graph. Results are expressed as mean ± SD.

Techniques: Injection

(A–D) Expression patterns of genes related to either osteoblast or osteoclast differentiation. (E) H&E (left) and TRAP chromogenic staining (right) were performed in trabecular bone of WT and IFT88-KOSF-1 littermates. Scale bars: 100 μm. (F) Number of osteoclasts per BS from E. (G) Amount of osteoclast surface per BS from E. (H and I) Relative expression of osteoblastogenic genes. (J) Serum P1NP levels of 12-week-old WT and IFT88-KOSF-1 mice. (K) Serum CTX-1 levels of 12-week-old WT and IFT88-KOSF-1 mice. Number of animals examined is expressed in parentheses in each graph. Data are expressed as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001, Student’s t test.

Journal: The Journal of Clinical Investigation

Article Title: Ventromedial hypothalamic primary cilia control energy and skeletal homeostasis

doi: 10.1172/JCI138107

Figure Lengend Snippet: (A–D) Expression patterns of genes related to either osteoblast or osteoclast differentiation. (E) H&E (left) and TRAP chromogenic staining (right) were performed in trabecular bone of WT and IFT88-KOSF-1 littermates. Scale bars: 100 μm. (F) Number of osteoclasts per BS from E. (G) Amount of osteoclast surface per BS from E. (H and I) Relative expression of osteoblastogenic genes. (J) Serum P1NP levels of 12-week-old WT and IFT88-KOSF-1 mice. (K) Serum CTX-1 levels of 12-week-old WT and IFT88-KOSF-1 mice. Number of animals examined is expressed in parentheses in each graph. Data are expressed as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001, Student’s t test.

Techniques: Expressing, Staining

(A) Calcein double labeling in cortical bone (upper) and trabecular bone (lower). Scale bar: 50 μm. (B and C), BFR (B) and MAR (C) of cortical bone of WT and IFT88-KOSF-1 mice. (D and E) BFR (D) and MAR (E) of trabecular bone of WT and IFT88-KOSF-1 mice. Ct, cortical bone; Tb, trabecular bone. Number of animals examined is expressed in parentheses in each graph. Data are expressed as mean ± SD. ***P < 0.001, Student’s t test.

Journal: The Journal of Clinical Investigation

Article Title: Ventromedial hypothalamic primary cilia control energy and skeletal homeostasis

doi: 10.1172/JCI138107

Figure Lengend Snippet: (A) Calcein double labeling in cortical bone (upper) and trabecular bone (lower). Scale bar: 50 μm. (B and C), BFR (B) and MAR (C) of cortical bone of WT and IFT88-KOSF-1 mice. (D and E) BFR (D) and MAR (E) of trabecular bone of WT and IFT88-KOSF-1 mice. Ct, cortical bone; Tb, trabecular bone. Number of animals examined is expressed in parentheses in each graph. Data are expressed as mean ± SD. ***P < 0.001, Student’s t test.

Techniques: Labeling

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